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Various Using Spectrophotometer on several Fields of Science


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A spectrophotometer is a scientific instrument commonly used in research and industrial laboratories. The instrument is often found in the fields of molecular biology, chemistry and biochemistry as it highly refers to production of Ultraviolet-Visible Rays. This light energy relies upon wavelengths that happen to be called lambda which may extend on the specific range. A typical human eye would usually answer wavelengths which extend 390 to 750 nanometers.

The spectrum associated with is comparable to the shades of rainbow which often defines colors that are readily interpreted by the brain. An optimal sensitivity for the eye is just about 555 nm which can be around the green portion of the optical spectrum. There are also colors which are not readily comprehended by the brain unless when combined multiple wavelengths. Unsaturated colors including pink, magenta or purple are great examples of this.

The visible window overlaps the human visible response spectrum. As the near infrared (NIR) spectrum is definitely away from human response window as well as the medium and long wavelength are way beyond the human region's response. Other species have the same function for visualizing colors like humans. Much like the bees as well as other insects, they can see ultraviolet rays that help them find nectars in flowers.

Hence, spectrophotometer is vital so that you can look into the spectrum regarding that objects emit and absorb. Example, when manufacturing computer monitors; NIR companies would blend red, green and blue of their respective color triangles. But blending of colors is hard advertising online seems since they have various wavelength i.e. blue is 450-474 nm.

Where are Spectrophotometer used?

The samples for Ultra violet rays are often measured in containers called cuvettes. This could be utilized if employed in the visible spectrum or ultraviolet ray measurements. NIR spectrophotometer is often used for industrial coloring specially in textile and ink manufacturers. Moreover, they are also employed in for color-sensitive materials used exclusively for forensics including printers.

As for biological and chemical research, solutions could be measured through the degree of light absorption at a specific wavelength. Example, molecular biology use NIR spectrometer to determine the energy DNA samples because of its compound mixture. This band are brilliant when enzymatic or chemical reactions cause to have the materials to alter overtime. Example of that is oxidation among citrus fruits. A spectrophotometer is useful in identifying how much oxidation accounts for the brownish influence on fruits.

Lastly, NIR reflectance is employed to confirm the spectroscopic method start using these materials. Moreover, reflectance is used to signal stretching or bending of chemical molecules like, nitrogen, carbon, hydrogen and oxygen. Such reflectance is especially useful when evaluating quality control of materials.


Lisun Electronics Inc was found by Lisun Group in 2003. We have sales & service offices in Shanghai, Russia, India and Korea. In 2012, we built a high level products show room & lab center in Shanghai. Lisun Group has set up a new manufactory in China to research and develop the world high-technology CFL & LED Test Instruments. Its quality system has been strictly certified by ISO9001:2008. The Lisun Group products have been authenticated by the third party lab and were awarded by CE certificate. As a CIE Supportive Member, all of the test instruments produced by Lisun Group are designed according to CIE Standards.

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